FastQCFastQC Report
Mon 2 May 2016
SRR3192657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3192657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93555584
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG101400.023.8385477
TAACACG160750.020.0594377
TAGTGCG189700.019.1411178-79
GTCTAAC172700.019.0272644
AACACGT183550.017.800648
GGCGCGT336700.017.5586225
GTACTCG292350.016.40631928-29
TACGCAC270050.015.87444950-51
TATGCCG250200.014.89912786-87
AGCGCTC357700.014.5115879
TCGTAAG111650.014.4191546
CGCGTGC436750.013.6026767
CCAGTAG411350.013.4024735
CGGTACA332450.013.382359560-61
ATCGCAC395250.013.31062268-69
TCTCCGG661000.013.2626116
TAGCATA437550.013.1874949
TGGCGCG438350.013.1727114
CGATCGC406100.013.10158566-67
TGCGAAT332250.013.04459374-75