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Protein IDs
One RefSeq accession (starting with XP / NP) or gi number per line (Limit 1000 proteins, please!).
[Sample format]
Paste in RefSeq accessions or gi numbers
-or-
Upload file containing RefSeq accessions or gi numbers
Parameters
Select sequences to include:
If you receive a warning that the sequences were not diverse enough for prediction on your substitution, try "all BLAST hits". If that fails, you can submit here and have SIFT find the appropriate number of sequences.
Remove sequences more than
percent identical to query
This cleans out any polymorphic alleles (sequences in the database that
are nearly identical to your
protein but already containing the substitutions of interest).
The contaminating sequences may cause the substitution to be predicted
as tolerated.