SIFT BLink TESTING only

SIFT BLink


SIFT BLink: Sequences are chosen from NCBI's precomputed BLAST searches. Either all BLAST hits can be used in the protein alignment or the top hit to each organism. Scoring is as described in Genome Research 12: 436-446.

This procedure is much faster than having us do the search. You can also submit your protein sequence and related sequences or aligned sequences if you already have them.

Results are deleted after an hour, so please save them!

[SIFT home page] [SIFT help] ]
Enter your email address if you want the results through email :
Please check that your address is correct and your mailbox is not full.




Protein sequence

NCBI GI number:
(Example: Type in 22209009 to get use the BLink sequences for gi:22209009)



Substitutions to be predicted on (can be left blank)
The positions of the substitutions must be relative to the protein sequence of the gi entered above.

Enter the substutions of interest [format]:
or enter the filename containing substitutions of interest [format]:




Parameters

Select sequences to include:
If you receive a warning that the sequences were not diverse enough for prediction on your substitution, try "all BLAST hits". If that fails, you can submit here and have SIFT find the appropriate number of sequences.

Remove sequences more than percent identical to query
This cleans out any polymorphic alleles (sequences in the database that are nearly identical to your protein but already containing the substitutions of interest). The contaminating sequences may cause the substitution to be predicted as tolerated.




Questions or comments?
Contact us
Last updated:
November 2008. Bug found where SIFT was not retrieving all the BLAST hits. Fixed 11/02/2005.